Amylase is present in human spit, where it begins the chemical procedure of digestion.
Different physicochemical conditions were varied for maximum enzyme production. It showed stability at pH range 5—7. It hydrolyses starch and is used commercially for the production of sugar syrups from starch which consist of glucose, maltose, and higher oligosaccharides Amylases are of great significance in biotechnological applications ranging from food, fermentation, detergent, pharmaceutical, brewing and textile to paper industries 18 To meet the higher demands of these industries, low cost production of amylase is required.
Amylase is produced in bacteria, fungi, plants and animals. However, due to efficient production strategies, microorganisms have substantial potential to contribute to a number of industrial applications Such industrially important microorganisms are found within the Bacillus species because of their rapid growth rates that lead to short fermentation cycles, their capacity to secrete proteins into extra cellular medium, and general handling safety However, the contents of a synthetic Research paper on amylase production are very expensive and uneconomical, so they need to be replaced with more economically available agricultural and industrial by-products, as they are considered to be good substrates for SSF to produce enzymes In recent years the technique of solid-state fermentation SSF process has been developed and used more extensively.
It has advantages over SmF like simple technique, low capital investment, cheaper production of enzyme having better physiochemical properties, lower levels of catabolite repression and better product recovery 5. The major factors that affect microbial synthesis of enzymes in a SSF system include selection of a suitable substrate and microorganism, particle size of the substrate, inoculum concentration and moisture level of the substrate.
Thus it involves the screening of a number of agro-industrial materials for microbial growth and product formation Temperature and pH are known to be important parameters in the production of enzymes from bacteria; hence, the thermal and the pH stability of the enzyme, which is a function of the exposure time, must also be taken into account.
The present work represents an investigation into amylase production by SSF with Wheat bran, Gram husk, Rice Bran and Mustard Oilseed cake as substrates and the determination of optimized production conditions. Screening of bacterial isolates Primary screening of bacterial isolates for production of alpha amylase was done by the starch agar plate method 2.
Out of 50 strains, the 3 strains that showed the biggest zone of clearance in starch hydrolysis were selected for production in Solid State Fermentation. The selected bacterial strains were inoculated in nutrient broth [consisting of g L SSF was performed with all the four substrates and their enzyme production was checked by assay.
Samples were aseptically withdrawn periodically and assayed for amylase activity.
Enzyme assay Estimation of amylase activity was carried out according to the DNSA 3, 5 dinitro salicylic acid method. The reaction was stopped by adding 2ml of DNS and kept in boiling water bath for 10min.
The absorbance was read at nm using a Spectrophotometre Shimadzu, Thermoelectric cell holder,Sagainst glucose as the standard. The experiments were carried out in triplicates and standard error was calculated.
Optimization studies for enzyme production In a sequential order, the various physicochemical factors as substrate, moisture content and inoculum size affecting the enzyme production were optimized for maximal enzyme production by using the solid substrate for which best amylase activity was observed.
Substrate The enzyme production was studied with all the four types of agro-industrial wastes viz. Moisture Moisture content for the solid state fermentation of the enzyme was evaluated by varying the moisture content from 1: Partial purification of enzyme Partially purified enzyme was obtained by addition of solid NH4 2SO4 to the crude enzyme obtained after solid state fermentation, with constant stirring at room temperature.
This precipitate was dissolved in 0. This dialysate was used as the enzyme solution.Full Length Research Paper - Production and partial characterization of a thermostable amylase from ascomycetes yeast strain isolated from starchy soils.
for the production of alpha amylase  because of the low cost of substrates used for the production of alpha amylases. Thus the present study was designed in search of cheaper carbon sources for the production of alpha amylase enzyme by fungal.
Dec 01, · INTRODUCTION. Amylase is one of the most widely used enzymes in the industry. It hydrolyses starch and is used commercially for the production of sugar syrups from starch which consist of glucose, maltose, and higher oligosaccharides ().Amylases are of great significance in biotechnological applications ranging from food, fermentation, detergent, pharmaceutical, brewing and textile to paper.
This report focuses on the microbial production of α-Amylase and its applications.
α-Amylase Production and Applications: A Review. there has been extensive research on microbial production of α-Amylase. There are 2 major reasons for the increasing interest in microbial sources: The role of α-Amylase in the paper industry is the.
Enzymes are very specific; for example, amylase is the only enzyme that will break down starch. It is similar to the theory of the lock and the key. The enzyme is the lock and the key is the substrate; only the correct key could fit into the keyhole of the lock.
Porcine pancreatic amylase is. Full length research paper Production of amylase from Aspergillus niger using a defined synthetic growth medium and also rice (Oryza sativa) as growth substrate amylase production studies have been done with mesophilic fungi within the temperature range of 25–37 °C (Francis et al., ).